About this document
Western Blot Protocol with 10x PBS by sms143 is a document available to read on EtoBox.
This document provides protocols for performing Western blots, including required solutions, cell lysis buffers, gel electrophoresis, protein transfer, antibody incubation, detection, stripping and reprobing membranes. Key steps include lysing cells in Laemmli buffer, running protein samples on an SDS-PAGE gel, transferring to a membrane, blocking the membrane, incubating with primary and secondary antibodies, developing with an ECL kit, and optionally stripping membranes for reprobing. Details are provided
- Author
- sms143
- Language
- EN