Can I read Differential effects of follicle-stimulating hormone, insulin, and insulin-like growth factor I on hexose uptake and lactate production by rat Sertoli cells on EtoBox?

Differential effects of follicle-stimulating hormone, insulin, and insulin-like growth factor I on hexose uptake and lactate production by rat Sertoli cells by Dr. Ria B. Oonk; Ruud Jansen; J. Anton Grootegoed is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Differential effects of follicle-stimulating hormone, insulin, and insulin-like growth factor I on hexose uptake and lactate production by rat Sertoli cells about?

The stimulatory effects of follicle-stimulating hormone (FSH), insulin, and insulinlike growth factor I (IGF-I) on lactate production and hexose uptake by Sertoli cells from immature rats were studied. The time-courses and the maximal stimulatory effects of FSH, insulin, and IGF-I on lactate production were virtually identical. When Sertoli cells were incubated in the presence of FSH in combination with insulin or IGF-I (submaximal doses), additive but no pronounced synergistic effects were observed. The stimulatory effects of FSH and insulin were not dependent on the presence of extracellular calcium. 2-Deoxy-D-glucose @DOG), an analogue of D-glucose, was used to investigate the hexose transport system of Sertoli cells. Uptake of 2-DOG was linear in time and virtually all of the intracellular 2-DOG was phosphorylated up to 30 min of incubation; 2-DOG uptake was inhibited by cytochalasin B, but not by cytochalasin E. 5-glucose, but not D-galactose, appeared to be an effective competitor of 2-DOG uptake. The K, , , of 2-DOG uptake was not influenced by FSH, insulin, and IGF-I. FSH had no effect on the V, , , , , of 2-DOG uptake, whereas insulin and IGF-I caused a 30% stimulation of

Who reads Differential effects of follicle-stimulating hormone, insulin, and insulin-like growth factor I on hexose uptake and lactate production by rat Sertoli cells?

Author

Dr. Ria B. Oonk; Ruud Jansen; J. Anton Grootegoed

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)