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Detection of five common variants of ABO gene by a triplex probe-based fluorescence-melting-curve-analysis 1 1 Introduction 1 2 Materials and methods 2 2.1 DNA samples 2 2.2 Triplex melting curve analysis for synthetic oligonucleotide templates 2 2.3 Triplex melting curve analysis for simultaneous genotyping of 261delG, 796C>A, 802G>A, 803G>C, and 1061delC 3 2.4 Sanger sequencing of PCR product 3 3 Results 3 3.1 Triplex melting curve analysis for synthetic oligonucleotide templates 3 3.2 Triplex melting curve analysis for genotyping of c.261delG, c.796C>A, c.802G>A, c.803G>C, and c.1061delC 3 3.3 Genotyping of five SNVs of ABO gene in Latin Americans of 1000 Genomes Project 4 3.4 Estimation of ABO phenotype from genotypes of five SNVs 4 4 Discussion 4 5 Conclusions 6 CRediT authorship contribution statement 6 Declaration of competing interest 6 Acknowledgments 7 Appendix A Supplementary data 7 References 7
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- Language
- EN
- Category
- nonfiction
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