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Reverse transcription of the pFOXC mitochondrial retroplasmids of Fusarium oxysporum is protein primed by Jeffrey T Galligan; Sarah E Marchetti; John C Kennell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background The pFOXC retroplasmids are small, autonomously replicating DNA molecules found in mitochondria of certain strains of the filamentous fungus __Fusarium oxysporum__ and are among the first linear genetic elements shown to replicate via reverse transcription. The plasmids have a unique clothespin structure that includes a 5'-linked protein and telomere-like terminal repeats, with pFOXC2 and pFOXC3 having iterative copies of a 5 bp sequence. The plasmids contain a single large open reading frame (ORF) encoding an active reverse transcriptase (RT). The pFOXC-RT is associated with the plasmid transcript in a ribonucleoprotein (RNP) complex and can synthesize full-length (-) strand cDNA products. In reactions containing partially purified RT preparations with exogenous RNAs, the pFOXC3-RT has been shown to initiate cDNA synthesis by use of snapped-back RNAs, as well as loosely associated DNA primers. ## Results The complete sequence of the distantly related pFOXC1 plasmid was determined and found to terminate in 3-5 copies of a 3 bp sequence. Unexpectedly, the majority of (-) strand cDNA molecules produced from endogenous pFOXC1 transcripts were attached to protein. __In vi

Who reads Reverse transcription of the pFOXC mitochondrial retroplasmids of Fusarium oxysporum is protein primed?

Author

Jeffrey T Galligan; Sarah E Marchetti; John C Kennell

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2010-; Springer Science and Business Media LLC (ISSN 1759-8753)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)