Can I read Purification and characterization of sago starch-degrading glucoamylase from Acremonium sp. endophytic fungus on EtoBox?

Purification and characterization of sago starch-degrading glucoamylase from Acremonium sp. endophytic fungus by Yetti Marlida; Nazamid Saari; Zaiton Hassan; Son Radu; Jamilah Bakar is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Purification and characterization of sago starch-degrading glucoamylase from Acremonium sp. endophytic fungus about?

A novel sago starch degrading glucoamylase which had a strong amylopectin-hydrolyzing-activity was puri®ed to homogeneity from a culture ®ltrate of Acremonium sp. isolated from forest trees. The puri®ed enzyme was an oligomeric protein of two sub-units with molecular weights of 22 and 39 kDa. Optimum temperature and pH for enzyme activity were around 55 C and 5.5, respectively. The enzyme was stable in a pH range of 3.0±7.0 and temperatures up to 60 C. The puri®ed enzyme was strongly inhibited by EDTA. The enzyme catalyzed hydrolysis of amylose and amylopectin, showed apparent K m values of 10.0 and 3.8 mg/ml and V max of 195 mmol/ml/min and 391 mmol/ml/min, respectively. Glucose was the sole product released by the hydrolysis, indicating that this enzyme displays an exo-action of starch-degrading activity.

Who reads Purification and characterization of sago starch-degrading glucoamylase from Acremonium sp. endophytic fungus?

Author

Yetti Marlida; Nazamid Saari; Zaiton Hassan; Son Radu; Jamilah Bakar

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)

Published

2000

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)