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Characterizing Medullary and Human Mesenchymal Stem Cell-derived Adipocytes by Danielle L. MacKay; Paul J. Tesar; Li-Nuo Liang; Stephen E. Haynesworth is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Characterizing Medullary and Human Mesenchymal Stem Cell-derived Adipocytes about?

## Abstract Throughout postnatal years, medullary adipocytes (MAs) increase in both number and size; however, knowledge of these cells pales in comparison to that of other adipocyte depots. It is widely hypothesized that MAs derive from multipotent progenitor cells of the bone marrow, such as human mesenchymal stem cells (hMSCs). Nevertheless, there is a paucity of comparative, molecular‐level studies in support of this hypothesis. In the present article, RTPCR was used to examine similarities and differences in gene expression among MAs, hMSC‐derived adipocytes, and subcutaneous adipocytes. While little or no message for lineage‐specific markers was detected in undifferentiated hMSCs, the data demonstrate that hMSC‐derived adipocytes, MAs, and subcutaneous adipocytes commonly express mRNA encoding for adipogenic transcription factors (PPARγ2, C/EBPα, and SREBP1), adipokines (adipsin, leptin, APM1, and angiotensinogen), and lipid‐metabolizing agents (aP2 and LPL), among other genes. None of the cell populations examined expressed a detectable level of the brown fat marker UCP1. This suggests highly similar gene expression between human subcutaneous and MAs, not previously substanti

Who reads Characterizing Medullary and Human Mesenchymal Stem Cell-derived Adipocytes?

Author

Danielle L. MacKay; Paul J. Tesar; Li-Nuo Liang; Stephen E. Haynesworth

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)