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Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures by Flona A. Redway; Vimla Vasil; Indra K. Vasil is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Stable cell suspension cultures were established from two types of calli (one compact, nodular and embryogenic, the other friable and embryogenic) derived from cultured immature embryos of wheat (cv FLA302). Only aged calli, which had been subcultured for at least 5-8 months, formed suspensions comprised mainly of groups of small, round, densely cytoplasmic, starch-containing cells. Only the embryogenic suspension derived from the aged, compact and nodular callus formed distinct somatic embryos when plated on regeneration media containing IAA and zeatin. Upon subsequent transfer to fresh regeneration medium more than 200 green rooted plants were obtained.

Who reads Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures?

Author

Flona A. Redway; Vimla Vasil; Indra K. Vasil

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0721-7714)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)