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Affinity Chromatography Explained by Anushree Sanjeevan is a document available to read on EtoBox.

Affinity chromatography is a purification technique that isolates biological molecules, such as proteins, by exploiting specific interactions between the target molecule and an immobilized ligand on a solid support. The process involves three main steps: binding, washing, and elution, which collectively yield highly purified products. While it offers high specificity and reproducibility, it also has drawbacks such as the cost of ligands and potential ligand leakage.

Author

Anushree Sanjeevan

Language

EN