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An immunogold single extracellular vesicular RNA and protein (^Au^SERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients by Luong T. H. Nguyen; Jingjing Zhang; Xilal Y. Rima; Xinyu Wang; Kwang Joo Kwak; Tamio Okimoto; Joseph Amann; Min Jin Yoon; Takehito Shukuya; Chi‐Ling Chiang; Nicole Walters; Yifan Ma; Donald Belcher; Hong Li; Andre F. Palmer; David P. Carbone; L. James Lee; Eduardo Reátegui is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is An immunogold single extracellular vesicular RNA and protein (^Au^SERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients about?
## Abstract Conventional PD‐L1 immunohistochemical tissue biopsies only predict 20%–40% of non‐small cell lung cancer (NSCLC) patients that will respond positively to anti‐PD‐1/PD‐L1 immunotherapy. Herein, we present an immunogold biochip to quantify single extracellular vesicular RNA and protein (^Au^SERP) as a non‐invasive alternative. With only 20 μl of purified serum, PD‐1/PD‐L1 proteins on the surface of extracellular vesicles (EVs) and EV PD‐1/PD‐L1 messenger RNA (mRNA) cargo were detected at a single‐vesicle resolution and exceeded the sensitivities of their bulk‐analysis conventional counterparts, ELISA and qRT‐PCR, by 1000 times. By testing a cohort of 27 non‐responding and 27 responding NSCLC patients, ^Au^SERP indicated that the single‐EV mRNA biomarkers surpass the single‐EV protein biomarkers in predicting patient responses to immunotherapy. Dual single‐EV PD‐1/PD‐L1 mRNA detection differentiated responders from non‐responders with an accuracy of 72.2% and achieved an NSCLC diagnosis accuracy of 93.2%, suggesting the potential for ^Au^SERP to provide enhanced immunotherapy predictions and cancer diagnoses within the clinical setting.
Who reads An immunogold single extracellular vesicular RNA and protein (^Au^SERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Luong T. H. Nguyen; Jingjing Zhang; Xilal Y. Rima; Xinyu Wang; Kwang Joo Kwak; Tamio Okimoto; Joseph Amann; Min Jin Yoon; Takehito Shukuya; Chi‐Ling Chiang; Nicole Walters; Yifan Ma; Donald Belcher; Hong Li; Andre F. Palmer; David P. Carbone; L. James Lee; Eduardo Reátegui
- Publisher
- Wiley
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)