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Expression of Neurotrophin Trk and P75 Receptors in Quail Embryos Undergoing Gastrulation and Neurulation by Damin Zhang; Lihua Yao; Paulette Bernd is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Expression of Neurotrophin Trk and P75 Receptors in Quail Embryos Undergoing Gastrulation and Neurulation about?

We have previously demonstrated the presence of mRNA for the full-length neurotrophin receptors trkA, trkB and trkC in quail embryos from stages 1 through 6 using reverse transcription followed by the polymerase chain reaction (RT-PCR Yao et al. 119941 Dev. Biol. 165 727-730). Furthermore, we showed that mRNA for the neurotrophins brain-derived neurotrophic factor and neurotrophin-3 was present from stage 1 onward, while nerve growth factor mRNA began to be expressed at stage 5. In the present study, wholemount in situ hybridization was used to localize full-length trk mRNA in embryos from stages 3 through 10. Structures expressing trkC mRNA included the primitive streak and Hensen's node, the neural plate or notochord, somites and the rostra1 neural tube. trkA and trkB mRNA were expressed at much lower levels than trkC mRNA; however, staining was detected on the primitive streak and Hensen's node. In addition to trk mRNA, we have also demonstrated the presence of full-length Trk protein in embryos from stages 3 through 11, suggesting that the trk mRNA detected at these early stages is translated into functional cell surface receptors. To support this hypothesis, we have shown that

Who reads Expression of Neurotrophin Trk and P75 Receptors in Quail Embryos Undergoing Gastrulation and Neurulation?

Author

Damin Zhang; Lihua Yao; Paulette Bernd

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)