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What is Chelex 100 DNA Extraction Protocol about?

This document summarizes a Chelex DNA extraction protocol for extracting DNA from modern tissue samples, including small pieces of dolphin skin or meat. The protocol involves adding a small piece of tissue to a tube containing Chelex resin and boiling for 20 minutes to lyse the cells and bind DNA to the Chelex beads. The supernatant containing the extracted DNA is then used as template for PCR amplification. The document also provides instructions for preparing aliquots of a 10% Chelex solution for multiple

Author

u77

Language

EN