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Practical considerations in the gas chromatography/combustion/isotope ratio monitoring mass spectrometry of ^13^C‐enriched compounds: detection limits and carryover effects by Hazel R. Mottram; Richard P. Evershed is a Chemistry article available to read on EtoBox.
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## Abstract This paper describes a methodological investigation of the use of gas chromatography/combustion/isotope ratio monitoring mass spectrometry (GC/C/IRMS) for the compound‐specific stable isotope analysis of ^13^C‐enriched compounds. Analysis of two ^13^C‐enriched fatty acid methyl esters, possessing __δ__^13^C values of ∼500‰, at a range of concentrations, demonstrated that detectable responses, i.e. chromatographic peaks, could be observed in the 45/44 output even when the compound was present in such low abundance that no peak was observed in the __m/z__ 44 ion chromatogram. A limit of detection, defined as the point at which the signal‐to‐background ratio was equal to 3, was calculated for two compounds and for both ion chromatograms. The limit of detection in the 45/44 chromatogram was found to be ca. 30 pg injected for methyl ^13^C‐hexadecanoate and ca. 20 pg injected for methyl ^13^C‐octadecanoate, whilst, in the __m/z__ 44 ion chromatogram, detection limits were ∼180 and ∼200 pg, respectively. The __δ__^13^C value recorded for the analytes was found to be both inaccurate and imprecise below 5 ng of each component injected, although this would not represent a signifi
Who reads Practical considerations in the gas chromatography/combustion/isotope ratio monitoring mass spectrometry of ^13^C‐enriched compounds: detection limits and carryover effects?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Hazel R. Mottram; Richard P. Evershed
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)