About this document
De Novo Protein Binding Pair Design by Sagar Khare is a document available to read on EtoBox.
The document describes research that used computational design and directed evolution to create a novel high-affinity protein-protein binding pair from two non-interacting proteins. Researchers developed a motif-based computational method to design protein interfaces with characteristics of natural interfaces, including high interaction density in the core. Applying this method, they designed a pair of proteins, Prb and Pdar, that bind with a Kd of 130 nM. Directed evolution identified mutations that improv
- Author
- Sagar Khare
- Language
- EN