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Immobilization of Horseradish Peroxidase on Amino-functionalized Carbon Dots for the Sensitive Detection of Hydrogen Peroxide by Ya Su; Xuan Zhou; Yumei Long; Weifeng Li is a Chemistry article available to read on EtoBox.

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The authors describe the preparation of amino-functionalized carbon dots (NH-CDs) via a one-step hydrothermal process using silver nitrate and chitosan as the precursors. The NH-CDs have a fairly consistent size distribution with an average size of 2.8 ± 0.5 nm. This is attributed to the introduction of Ag(I) both as a catalyst and a precipitant. The NH-CDs are highly crystalline. Their surface carries amino groups and carboxy groups which is confirmed by transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Horseradish peroxidase (HRP) was immobilized in the NH-CDs and then placed on a glassy carbon electrode (GCE). Spectroscopic and electrochemical analyses evidenced the stability and good bioactivity of the immobilized HRP. This reveals that NH-CD is a desirable matrix for enzyme immobilization. The modified GCE exhibits enhanced electro-catalytic activity towards hydrogen peroxide (HO) reduction as compared to that of plain CDs. The effects of pH value and loading on the performances of the modified GCEs were studied. Under optimized conditions, the biosensor has a linear response in the 5 to 590 nM HO concentration range, with a 1.8 nM defe

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Author

Ya Su; Xuan Zhou; Yumei Long; Weifeng Li

Publisher

Springer Science and Business Media LLC

Published

2018

Language

EN

Field

Chemistry (Physical Sciences)