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A Ca^2+^/CaM‐dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter by Sona Pandey; Shiv B. Tiwari; Wricha Tyagi; Mali K. Reddy; Kailash C. Upadhyaya; Sudhir K. Sopory is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A Ca^2+^/CaM‐dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter about?
An immuno‐homologue of maize Ca^2+^/calmodulin (CaM)‐dependent protein kinase with a molecular mass of 72 kDa was identified in pea. The pea kinase (PsCCaMK) was upregulated in roots in response to low temperature and increased salinity. Exogenous Ca^2+^ application increased the kinase level and the response was faster than that obtained following stress application. Low temperature‐mediated, but not salinity‐mediated stress kinase increase was inhibited by the application of EGTA and W7, a CaM inhibitor. The purification of PsCCaMK using immuno‐affinity chromatography resulted in coelution of the kinase with another polypeptide of molecular mass 40 kDa (p40). Western blot revealed the presence of PsCCaMK in nuclear protein extracts and was found to phosphorylate p40 __in vitro__. Gel mobility shift and South‐Western analysis showed that p40 is a DNA‐binding protein and it interacted specifically with one of the __cis__ acting elements of the __Arabidopsis__ CaM5 gene (__AtCaM5__) promoter. The binding of p40 to the specific elements in the __AtCaM5__ promoter was dependent of its dephosphorylated state. Our results suggest that p40 could be an upstream signal component of the str
Who reads A Ca^2+^/CaM‐dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sona Pandey; Shiv B. Tiwari; Wricha Tyagi; Mali K. Reddy; Kailash C. Upadhyaya; Sudhir K. Sopory
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)