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Biochemical isolation and purification of ovulation-inducing factor (OIF) in seminal plasma of llamas by Marcelo H Ratto; Louis TJ Delbaere; Yvonne A Leduc; Roger A Pierson; Gregg P Adams is a Medicine article available to read on EtoBox.

What is Biochemical isolation and purification of ovulation-inducing factor (OIF) in seminal plasma of llamas about?

## Background The objective of the present study was to isolate and purify the protein fraction(s) of llama seminal plasma responsible for the ovulation-inducing effect of the ejaculate. ## Methods Semen collected from male llamas by artificial vagina was centrifuged and the seminal plasma was harvested and stored frozen. Seminal plasma was thawed and loaded onto a Type 1 macro-prep ceramic hydroxylapatite column and elution was carried out using a lineal gradient with 350 mM sodium phosphate. Three protein fractions were identified clearly (Fractions A, B, and C), where a prominent protein band with a mass of 14 kDa was identified in Fraction C. Fraction C was loaded into a sephacryl gel filtration column for further purification using fast protein liquid chromatography (FPLC). Isocratic elution resulted in 2 distinct protein fractions (Fractions C1 and C2). An in vivo bioassay (n = 10 to 11 llamas per group) was used to determine the ovarian effect of each fraction involving treatment with saline (negative control), whole seminal plasma (positive control), or seminal plasma Fractions A, B or C2. Ultrasonography was done to detect ovulation and CL formation, and blood samples were

Who reads Biochemical isolation and purification of ovulation-inducing factor (OIF) in seminal plasma of llamas?

Author

Marcelo H Ratto; Louis TJ Delbaere; Yvonne A Leduc; Roger A Pierson; Gregg P Adams

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2003-; Springer Science and Business Media LLC (ISSN 1477-7827)

Published

2011

Language

EN

Field

Medicine (Health Sciences)