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A New Spectrophotometric Method for the Determination of 5′-Nucleotidase by F. Heinz; Renate Pilz; Sylvia Reckel; J. R. Kalden; R. Haeckel is a Medicine article available to read on EtoBox.

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A Spectrophotometric method is described for the determination of 5'-nucleotidase. In combination with the enzymes nucleoside phosphorylase and xanthine oxidase, inosine, formed by hydrolysis of S'-IMP by S'-nucleotidase, is cleaved phosphorolytically to hypoxanthine, which is oxidized to uric acid. In the presence of ethanol, the hydrogen peroxide formed is reduced by catalase and equivalent amounts of acetaldehyde are produced. The aldehyde is dehydrogenated (NADP-dependent) by aldehyde dehydrogenase and the production ι rate of NADPH is recorded at 334 nm. The inhibition of the unspecific cleavage of 5'-IMP by phosphatases is examined critically.

Who reads A New Spectrophotometric Method for the Determination of 5′-Nucleotidase?

Author

F. Heinz; Renate Pilz; Sylvia Reckel; J. R. Kalden; R. Haeckel

Publisher

Walter de Gruyter GmbH

Published

1980

Language

EN

Field

Medicine (Health Sciences)