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EPR evidence for superoxide anion formation in leaves during exposure to low levels of ozone by V. C. RUNECKLES; M. VAARTNOU is a Agricultural and Biological Sciences article available to read on EtoBox.

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## ABSTRACT Although the superoxide anion radical (O) has been implicated in the phytotoxicity of ozone, (O~3~), its role has been inferred from indirect evidence based on the activity of oxyradical scavenging systems in the leaf, particularly superoxide dismutase (SOD). Direct observations of radical signals obtained by electron paramagnetic resonance spectrometry (EPR) of intact, attached leaves of bluegrass (__Poa pratensis__ L.) and ryegrass (__Lolium perenne__ L.) and leaf pieces of radish (__Raphanus sativus__ L.) during exposure to 240 μg m^−3^ O~3~ in air flowing through the spectrometer cavity have revealed the appearance of a signal with the characteristics of O. The exposures used were insufficient to cause any necrotic injury to the leaves. The appearance of the signal is light‐dependent, suggesting that it originates in the chloroplast, and its appearance is reduced in leaves in which the apoplastic pool of ascorbic acid has been enriched by prior vacuum infiltration. In each species, the signal only appeared after about 1 h of exposure to O~3~, and then increased steadily over the next 4 h. The lability of the species responsible for the signal is such that it can no

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Author

V. C. RUNECKLES; M. VAARTNOU

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0140-7791)

Published

1997

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)