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Differential Processing of Osteopontin Characterizes the Proliferative Vascular Smooth Muscle Cell Phenotype Induced by Allylamine by Alan R. Parrish; Kenneth S. Ramos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Repeated cycles of vascular injury by allylamine induce vascular lesions similar to those seen in atherosclerotic vessels, or following balloon catheterization. Vascular (aortic) smooth muscle cells harvested from allylamine-treated animals (i.e., allylamine cells) acquire a proliferative advantage relative to control counterparts that is associated with differential secretion and extracellular matrix sequestration of several proteins. In the present study, we have characterized two of these proteins (M r 52 and 36 kDa; pI 5.6 and 5.2, respectively) and their putative role in the expression of a proliferative phenotype. Because the physical properties of these proteins were comparable to those of osteopontin (OPN) and its thrombin-generated fragment(s), initial experiments were conducted to examine the expression and processing of OPN in this cell system. OPN mRNA expression was enhanced during early G 1 cell cycle progression in allylamine cells relative to control counterparts. However, comparable amounts of OPN (M r 56, 52, and 50 kDa) were detected by Western analysis in media conditioned by both cell types using the OP-199 or B77-Rat1 antibodies to OPN. Allylamine cells, howev

Who reads Differential Processing of Osteopontin Characterizes the Proliferative Vascular Smooth Muscle Cell Phenotype Induced by Allylamine?

Author

Alan R. Parrish; Kenneth S. Ramos

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)