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Use of High‐resolution Mass Spectrometry to Investigate a Metabolite Interference During Liquid Chromatography/tandem Mass Spectrometric Quantification of a Small Molecule in Toxicokinetic Study Samples by Michael Furlong; Andrew Bessire; Wei Song; Christopher Huntington; Elizabeth Groeber is a Chemistry article available to read on EtoBox.
What is Use of High‐resolution Mass Spectrometry to Investigate a Metabolite Interference During Liquid Chromatography/tandem Mass Spectrometric Quantification of a Small Molecule in Toxicokinetic Study Samples about?
## Abstract During routine liquid chromatography/tandem mass spectrometric (LC/MS/MS) bioanalysis of a small molecule analyte in rat serum samples from a toxicokinetic study, an unexpected interfering peak was observed in the extracted ion chromatogram of the internal standard. No interfering peaks were observed in the extracted ion chromatogram of the analyte. The dose‐dependent peak area response and peak area response versus time profiles of the interfering peak suggested that it might have been related to a metabolite of the dosed compound. Further investigation using high‐resolution mass spectrometry led to unequivocal identification of the interfering peak as an __N__‐desmethyl metabolite of the parent analyte. High‐resolution mass spectrometry (HRMS) was also used to demonstrate that the interfering response of the metabolite in the multiple reaction monitoring (MRM) channel of the internal standard was due to an isobaric relationship between the ^13^C‐isotope of the metabolite and the internal standard (i.e., common precursor ion mass), coupled with a metabolite product ion with identical mass to the product ion used in the MRM transition of the internal standard. These res
Who reads Use of High‐resolution Mass Spectrometry to Investigate a Metabolite Interference During Liquid Chromatography/tandem Mass Spectrometric Quantification of a Small Molecule in Toxicokinetic Study Samples?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Michael Furlong; Andrew Bessire; Wei Song; Christopher Huntington; Elizabeth Groeber
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2010
- Language
- EN
- Field
- Chemistry (Physical Sciences)