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Can I read 3D correlative electron microscopy reveals continuity of Brucella -containing vacuoles with the endoplasmic reticulum on EtoBox?
3D correlative electron microscopy reveals continuity of Brucella -containing vacuoles with the endoplasmic reticulum by Jaroslaw Sedzicki; Therese Tschon; Shyan Huey Low; Kevin Willemart; Kenneth N. Goldie; Jean-Jacques Letesson; Henning Stahlberg; Christoph Dehio is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is 3D correlative electron microscopy reveals continuity of Brucella -containing vacuoles with the endoplasmic reticulum about?
Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers, however, the relationship of the vacuole to the genuine ER has remained elusive. We have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion beam/scanning electron microscopic tomography (FIB/SEM). Studying B. abortus-infected HeLa cells and trophoblasts derived from B. melitensis-infected mice, we demonstrate that rBCVs are complex a
Who reads 3D correlative electron microscopy reveals continuity of Brucella -containing vacuoles with the endoplasmic reticulum?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jaroslaw Sedzicki; Therese Tschon; Shyan Huey Low; Kevin Willemart; Kenneth N. Goldie; Jean-Jacques Letesson; Henning Stahlberg; Christoph Dehio
- Publisher
- The Company of Biologists
- Published
- 2018
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)