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Proliferation and differentiation of rat adipose precursor cells in chemically defined medium: Differential action of anti-adipogenic agents by Georges Vassaux; Raymond Négrel; Gérard Ailhaud; Danielle Gaillard is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Primary rat adipose precursor cells, maintained in the minimal chemically defined medium (ITT medium) able to promote differentiation, have been used to investigate the ability of several agents to modulate their proliferation and their differentiation. Fetuin and fibroblast growth factor (FGF), which exhibited a strong and a weak mitogenic activity, respectively, do not significantly affect the proportion of differentiated cells as indicated by glycerol-3-phosphate dehydrogenase (GPDH) activity values. In contrast, carbaprostacyclin (cPGI,), a stable analogue of prostacyclin, behaves as a true adipogenic factor leading to a 4 to 5-fold increase in GPDH-specific activities with no significant effect on cell growth. Submaxillary gland kallikrein (SMGK), transforming growth factor-6 (TCF-P) and tumor necrosis factor-a (TNF-a) behave as growth-promoting agents but at the same time elicit a dose-dependent inhibition of differentiation. Epidermal growth factor (EGF) and prostaglandin F,, (PGF, , ) do not show any effect on cell proliferation at concentrations which exert a maximal inhibitory action on differentiation. Upon removal of EGF from the culture medium, complete resumption of d

Who reads Proliferation and differentiation of rat adipose precursor cells in chemically defined medium: Differential action of anti-adipogenic agents?

Author

Georges Vassaux; Raymond Négrel; Gérard Ailhaud; Danielle Gaillard

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)