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Identification of hemocyanin as a novel non-cross-reactive allergen from the giant freshwater shrimp Macrobrachium rosenbergii by Surapon Piboonpocanun; Orathai Jirapongsananuruk; Thitinun Tipayanon; Siribangon Boonchoo; Richard E. Goodman is a Medicine article available to read on EtoBox.

What is Identification of hemocyanin as a novel non-cross-reactive allergen from the giant freshwater shrimp Macrobrachium rosenbergii about?

## Scope: Sensitization to giant freshwater shrimp macrobrachium rosenbergii (mr) was recently reported. however, the allergens have yet to be identified. this study aimed to identify and characterize a novel allergen of mr shrimp. ## Methods and results: Extracted proteins were separated and purified by anion and in some experiments, size-exclusion chromatography. serum ige from shrimp allergic donors identified a candidate protein, which was characterized by lc-ms/ms. the specificity of ige binding was tested using immunoblotting and inhibition elisa. the ige-binding profiles from 12 of 13 mr allergic subjects that were pre-incubated with an extract of penaeus monodon showed residual binding to ~60-80 kda proteins. the 60-80 kda ige-bound proteins were fractionated in the flow-through of anion chromatography showing a high ige reactivity. peptides identified by lc-ms/ms showed the proteins closely match subunits of hemocyanin (hcs). purified hcs from hemolymph markedly inhibited binding of ige from sera of mr allergic subjects to solid-phased mr proteins in inhibition elisa. ## Conclusion: Hcs were identified as heat-stable, non-cross-reactive, high-molecular-weight (mw) allergen

Who reads Identification of hemocyanin as a novel non-cross-reactive allergen from the giant freshwater shrimp Macrobrachium rosenbergii?

Author

Surapon Piboonpocanun; Orathai Jirapongsananuruk; Thitinun Tipayanon; Siribangon Boonchoo; Richard E. Goodman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1613-4125)

Published

2011

Language

EN

Field

Medicine (Health Sciences)