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Can I read Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films on EtoBox?
Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films by Saengrawee Sriwichai; Akira Baba; Sukon Phanichphant; Kazunari Shinbo; Keizo Kato; Futao Kaneko is a Engineering article available to read on EtoBox.
What is Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films about?
An electrochemically controlled surface plasmon resonance (SPR) immunosensor based on carboxylated polyaniline was constructed for detecting human immunoglobulin G (IgG) without using further labeled molecules. The carboxylated polyaniline, poly(3-aminobenzoic acid) (PABA), was prepared by electropolymerization of its monomer, 3-aminobenzoic acid (ABA), on a gold-coated high-refractiveindex glass slide that was also used as a working electrode for the electrochemical system. The kinetic properties during electropolymerization were monitored in situ with electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. The obtained PABA film, which was used to construct the immunosensor, showed electroactivity in neutral phosphate-buffered saline solution. The carboxylic acid surface of the PABA film was then activated to immobilize anti-human IgG. Finally, the immunosensor electrode was used to probe the binding reaction of anti-human/human IgG with various concentrations of human IgG at different constant applied potentials. The immobilization and binding reaction processes were controlled and also studied in situ by EC-SPR spectroscopy. The binding process at different applied pot
Who reads Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Saengrawee Sriwichai; Akira Baba; Sukon Phanichphant; Kazunari Shinbo; Keizo Kato; Futao Kaneko
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0925-4005)
- Published
- 2010
- Language
- EN
- Field
- Engineering (Physical Sciences)