Opening book details…
Can I read Increasing nitrogenase catalytic efficiency for MgATP by changing serine 16 of its Fe protein to threonine: Use of Mn^2+^ to show interaction of serine 16 with Mg^2+^ on EtoBox?
Increasing nitrogenase catalytic efficiency for MgATP by changing serine 16 of its Fe protein to threonine: Use of Mn^2+^ to show interaction of serine 16 with Mg^2+^ by Lance C. Seefeldt; Leonard E. Mortenson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Increasing nitrogenase catalytic efficiency for MgATP by changing serine 16 of its Fe protein to threonine: Use of Mn^2+^ to show interaction of serine 16 with Mg^2+^ about?
## Abstract MgATP‐binding and hydrolysis are an integral part of the nitrogenase catalytic mechanism. We are exploring the function of MgATP hydrolysis in this reaction by analyzing the properties of the Fe protein (FeP) component of __Azotobacter vinelandii__ nitrogenase altered by site‐directed mutagenesis. We have previously (Seefeldt, L.C., Morgan, T.V., Dean, D.R., & Mortenson, L.E., 1992, __J. Biol. Chem. 267__, 6680–6688) identified a region near the N‐terminus of FeP that is involved in interaction with MgATP. This region of FeP is homologous to the well‐known nucleotide‐binding motif GXXXXGKS/T. In the present work, we examined the function of the four hydroxyl‐containing amino acids immediately C‐terminal to the conserved lysine 15 that is involved in interaction with the β‐phosphate of MgATP. We have established, by altering independently Thr 17, Thr 18, and Thr 19 to alanine, that a hydroxyl‐containing residue is not needed at these positions for FeP to function. In contrast, an hydroxyl‐containing amino acid at position 16 was found to be critical for FeP function. When the strictly conserved Ser 16 was altered to Ala, Cys, Asp, or Gly, the FeP did not support N~2~ fix
Who reads Increasing nitrogenase catalytic efficiency for MgATP by changing serine 16 of its Fe protein to threonine: Use of Mn^2+^ to show interaction of serine 16 with Mg^2+^?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Lance C. Seefeldt; Leonard E. Mortenson
- Publisher
- Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)
- Published
- 1993
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)