Can I read Desulfotomaculum genus‐ and subgenus‐specific 16S rRNA hybridization probes for environmental studies on EtoBox?

Desulfotomaculum genus‐ and subgenus‐specific 16S rRNA hybridization probes for environmental studies by Krassimira R. Hristova; Margit Mau; Dandan Zheng; Rustam I. Aminov; Roderick I. Mackie; H. Rex Gaskins; Lutgarde Raskin is a Environmental Science article available to read on EtoBox.

What is Desulfotomaculum genus‐ and subgenus‐specific 16S rRNA hybridization probes for environmental studies about?

Based on comparative analysis of 16S rRNA sequences and the recently established phylogeny of the genus __Desulfotomaculum__, a set of phylogenetically nested hybridization probes was developed and characterized. A genus‐specific probe targets all known __Desulfotomaculum__ species (with the exception of __Desulfotomaculum acetoxidans__ ), and five specific probes target subclusters within the __Desulfotomaculum__ genus. The dissociation temperature of each probe was determined experimentally. Probe specificities were verified through hybridizations with pure culture rRNA isolated from a wide variety of target and non‐target organisms and through an evaluation of probe ‘nesting’ using samples obtained from four different environments. Fixation and hybridization conditions for fluorescence __in situ__ hybridizations were also optimized. The probes were used in quantitative membrane hybridizations to determine the abundance of __Desulfotomaculum__ species in thermophilic anaerobic digesters, in soil, in human faeces and in pig colon samples. __Desulfotomaculum__ rRNA accounted for 0.3–2.1% of the total rRNA in the digesters, 2.6–6.6% in soil, 1.5–3.3% in human faeces and 2.5–6.2% in

Who reads Desulfotomaculum genus‐ and subgenus‐specific 16S rRNA hybridization probes for environmental studies?

Author

Krassimira R. Hristova; Margit Mau; Dandan Zheng; Rustam I. Aminov; Roderick I. Mackie; H. Rex Gaskins; Lutgarde Raskin

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1462-2912)

Published

2000

Language

EN

Field

Environmental Science (Physical Sciences)