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Expression of antisense nodulin-35 RNA in Vigna aconitifolia transgenic root nodules retards peroxisome development and affects nitrogen availability to the plant by Na-Gyong Lee; Barry Stein; Hideki Suzuki; Desh Pal S. Verma is a Agricultural and Biological Sciences article available to read on EtoBox.

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A nodulin‐35 (N‐35) cDNA encoding nodule‐specific uricase (EC 1.7.3.3.) was isolated from a __Vigna aconitifolia__ (mothbean) root nodule cDNA library. Sequence analysis of __Vigna__ uricase (VN‐35) cDNA revealed 90% homology to that of soybean. The VN‐35 cDNA was inserted in the antisense orientation downstream of the CaMV—35S promoter, and transgenic hairy roots were formed on __Vigna__ plants using __Agrobacterium rhizogenes__. Infection with __Bradyrhizobium__ (cowpea) gave rise to root nodules on transgenic hairy roots supported by the wild‐type shoot. Expression of antisense VN‐35 RNA was detected in transgenic nodules on individual roots using polymerase chain reaction (PCR). The nodules expressing antisense VN‐35 RNA were smaller in size and showed lower uricase activity than nodules formed on the hairy roots transformed with a binary vector containing β‐glucuronidase (GUS) gene (used as control), and the plants exhibited nitrogen deficiency symptoms. Ultrastructural analysis and immunogold labeling with antibody against soybean N‐35 revealed that the growth of peroxisomes was retarded in transgenic nodules expressing antisense VN‐35 RNA. These data suggest that a reduction

Who reads Expression of antisense nodulin-35 RNA in Vigna aconitifolia transgenic root nodules retards peroxisome development and affects nitrogen availability to the plant?

Author

Na-Gyong Lee; Barry Stein; Hideki Suzuki; Desh Pal S. Verma

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)