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Comparison of the formation of benzo[a]pyrene diolepoxide-dna adducts in vitro by rat and human microsomes: Evidence for the involvement of p-450IA1 and p-450IA2 by Dr Kirsi Vähäkangas; Hannu Raunio; Markku Pasamen; Päivi Sivonen; Sang-Shin Park; Harry V. Gelboin; Olavi Pelkonen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Comparison of the formation of benzo[a]pyrene diolepoxide-dna adducts in vitro by rat and human microsomes: Evidence for the involvement of p-450IA1 and p-450IA2 about?
The involvement of cytochrome P-450 isozymes in the activation of benzo[a]pyrene (BP) by human placental and liver microsomes was studied in vitro using monoclonal antibodies (Mab) toward the major 3-methylcholanthrene (MC)-inducible and phenobarbital-inductible rat liver P-450 isozymes (Mab 1-7-1 and Mab 2-66-3, respectively). Microsomes from human placenta and liver and rat liver were incubated with BP and DNA, and BP-diolepoxide-DNA (BPDE-DNA) adducts were measured by synchronous fluorescence spectrophotometry (SFS). The only BP metabolite giving the same fluorescence peak as chemically modified BPDE-DNA was BP-7,8-dihydrodiol. Five (smokers) out of 29 human placentas (smokers and nonsmokers), and five out of nine human livers were able to metabolically activate BP to BPDE-DNA adducts in this system. The Mab 1-7-1 totally inhibited the formation of BPDE-DNA adducts in placental microsomal incubations. Inhibition using rat or human liver microsomes was 50-60°/0 and about 90%, respectively. The Mab 2-66-3 had no effect in any of the microsome types. Adduct formation was inhibited more strongly and at lower concentrations of Mab 1-7-1 compared with the inhibition of AHH activity. T
Who reads Comparison of the formation of benzo[a]pyrene diolepoxide-dna adducts in vitro by rat and human microsomes: Evidence for the involvement of p-450IA1 and p-450IA2?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Dr Kirsi Vähäkangas; Hannu Raunio; Markku Pasamen; Päivi Sivonen; Sang-Shin Park; Harry V. Gelboin; Olavi Pelkonen
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); VCH Publishers; Wiley (ISSN 0887-2082)
- Published
- 1989
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)