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X-ray Tomographic Reconstruction of Macromolecular Samples by Sandor Brockhauser; Marco Di Michiel; John E. McGeehan; Andrew A. McCarthy; Raimond B. G. Ravelli is a Materials Science article available to read on EtoBox.

What is X-ray Tomographic Reconstruction of Macromolecular Samples about?

The anomalous scattering properties of innate sulfur for proteins and phosphorus for DNA and RNA can be used to solve the phase problem in macromolecular crystallography (MX)__via__the single-wavelength anomalous dispersion method (SAD). However, this method, which is carried out at longer X-ray wavelengths (1.5–2.5 Å), is still not a routine tool in MX. The increased absorption from both air and sample associated with the use of longer X-ray wavelengths presents a key difficulty. The absorption can be corrected for through empirical algorithms, provided truly redundant data are available. Unfortunately, weakly diffracting macromolecular crystals suffer from radiation damage, resulting in a dose-dependent non-isomorphism which violates the assumption upon which these empirical algorithms are based. In this report, X-ray microtomography is used to reconstruct the three-dimensional shapes of vitrified macromolecular crystals including the surrounding solvent and sample holder. The setup can be integrated within an MX beamline environment and exploits both absorption and phase contrast. The dose needed for the tomographic measurements could be low enough to allow the technique to be u

Who reads X-ray Tomographic Reconstruction of Macromolecular Samples?

Author

Sandor Brockhauser; Marco Di Michiel; John E. McGeehan; Andrew A. McCarthy; Raimond B. G. Ravelli

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0021-8898)

Published

2008

Language

EN

Field

Materials Science (Physical Sciences)