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The ionization of a buried glutamic acid is thermodynamically linked to the stability of Leishmania mexicana triose phosphate isomerase by Anne-Marie Lambeir; Jan Backmann; Javier Ruiz-Sanz; Vladimir Filimonov; Jens Erik Nielsen; Inari Kursula; Brian V. Norledge; Rik K. Wierenga is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The ionization of a buried glutamic acid is thermodynamically linked to the stability of Leishmania mexicana triose phosphate isomerase about?
The amino acid sequence of __Leishmania mexicana__ triose phosphate isomerase is unique in having at position 65 a glutamic acid instead of a glutamine. The stability properties of LmTIM and the E65Q mutant were investigated by pH and guanidinium chloride‐induced unfolding. The crystal structure of E65Q was determined. Three important observations were made: (a) there are no structural rearrangements as the result of the substitution; (b) the mutant is more stable than the wild‐type; and (c) the stability of the wild‐type enzyme shows strong pH dependence, which can be attributed to the ionization of Glu65. Burying of the Glu65 side chain in the uncharged environment of the dimer interface results in a shift in p__K__~a~ of more than 3 units. The pH‐dependent decrease in overall stability is due to weakening of the monomer–monomer interactions (in the dimer). The E65Q substitution causes an increase in stability as the result of the formation of an additional hydrogen bond in each subunit (ΔΔ__G__ ° of 2 kcal·mol^−1^ per monomer) and the elimination of a charged group in the dimer interface (ΔΔ__G__ ° of at least 9 kcal·mol^−1^ per dimer). The computated shift in p__K__~a~ and the
Who reads The ionization of a buried glutamic acid is thermodynamically linked to the stability of Leishmania mexicana triose phosphate isomerase?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Anne-Marie Lambeir; Jan Backmann; Javier Ruiz-Sanz; Vladimir Filimonov; Jens Erik Nielsen; Inari Kursula; Brian V. Norledge; Rik K. Wierenga
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)