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Quantitative Determination of Captopril in Blood and Captopril and Its Disulfide Metabolites in Plasma by Gas Chromatography by M. S. Bathala; S. H. Weinstein; F. S. Meeker Jr; S. M. Singhvi; B. H. Migdalof is a Chemistry article available to read on EtoBox.

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A sensitive, quantitative gas chromatographic-electron capture (GC-EC) method for the determination of captopril in blood and captopril and its disulfide metabolites (collectively) in plasma was developed. After addition of an internal standard and N-ethylmaleimide to the biological samples, excess N-ethylmaleimide and naturally occurring interfering substances were removed by extraction with benzene followed by acidification and extraction with hexane. The N-ethylmaleimide adducts of captopril and of the internal standard were then extracted with benzene and converted to their hexafluoroisopropyl esters. For the assay of captopril and its disulfide metabolites, tributylphosphine was used to reduce the disulfide metabolites to captopril prior to derivatization. The hexafluoroisopropyl esters of the N-ethylmaleimide adducts of captopril and of the internal standard, the 4-ethoxyproline analogue of captopril, were separated by GC on a column packed with 3% OV-101 on Chromosorb W-HP. The lower limits of sensitivity were 20 ng/mL for captopril in blood and 50 ng/mL for captopril and its disulfide metabolites in plasma. Linearity, precision, and accuracy were excellent. The method was v

Who reads Quantitative Determination of Captopril in Blood and Captopril and Its Disulfide Metabolites in Plasma by Gas Chromatography?

Author

M. S. Bathala; S. H. Weinstein; F. S. Meeker Jr; S. M. Singhvi; B. H. Migdalof

Publisher

John Wiley and Sons; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0022-3549)

Published

1984

Language

EN

Field

Chemistry (Physical Sciences)