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Determination of quantum yield of NADH and FAD in alcohol-water solutions: the analysis of radiative and nonradiative relaxation pathways by Gorbunova, Ioanna A.; Danilova, Marina K.; Sasin, Maxim E.; Belik, Victor P.; Golyshev, Dmitrii P.; Vasyutinskii, Oleg S. is a scholarly article available to read on EtoBox.

What is Determination of quantum yield of NADH and FAD in alcohol-water solutions: the analysis of radiative and nonradiative relaxation pathways about?

Combined studies on fluorescence quantum yield in coenzymes NADH and FAD in water-methanol, water-ethanol and water-propylene glycol mixtures and on time-resolved fluorescence of the same molecules under excitation at 450 and 355~nm by means of the TCSPC method have been carried out. The dependence of quantum yield in NADH on alcohol concentration was found to be similar in methanol and ethanol and different from that in propylene glycol. The behavior of quantum yield in FAD was found to be almost independent of the type of alcohol and exhibited a dramatic 5--6 times increase with alcohol concentration. A model describing molecular excited state relaxation dynamics in pico- and nanosecond time domain was developed on the basis on the quantum mechanical theory and used for analysis of the experimental results. In particular, the model provided a new insight into the nature of the Decay Associated Spectra (DAS) phenomenon. The analysis of the role of pico- and nanosecond quenching in NADH suggested that the picosecond decay in methanol and ethanol likely does not occur through electron transfer in the stacking configuration of the nicotinamide and adenine moieties, but through other

Author

Gorbunova, Ioanna A.; Danilova, Marina K.; Sasin, Maxim E.; Belik, Victor P.; Golyshev, Dmitrii P.; Vasyutinskii, Oleg S.

Published

2022

Language

EN