Can I read A20 on EtoBox?

A20 by He Li; Congpin Wang; Xiaoqing Li; Yinghui Kong; Weiguo Sun is a Medicine article available to read on EtoBox.

What is A20 about?

Melanocyte-specific CD8 T cells enrichment correlates with the severity of vitiligo, and the role of A20 derived from myeloid cells in the enrichment of pathogenic T cells is unknown. Premelanosome (PMEL)-specific transgenic CD8 T cells were adoptive transferred into Krt14-Kitl\* mice to construct the vitiligo model, which was further mated with A20 mice and IKK2 mice. Bone marrow cells were stimulated with 30% L929 cell-conditioned medium, Fc-human tumor necrosis factor, and lipopolysaccharides to induce bone marrow-derived macrophages (BMDMs). The relative expression of CCL2, CCL5, and IL12A was detected with real-time PCR, and nuclear factor kappa B (NFκB) related molecules were detected with Western blots. Fluorescence-activated cell sorting (FACS) was utilized to assay the percent of innate and adaptive immune cells in the spleen and bone marrow, and CD45 T in the skin. Down-regulated A20 was detected in the skin biopsies of vitiligo patients. A20 deficiency did not affect the development of T cells, B cells, macrophages, and neutrophils. A20 negatively regulated the induction of proinflammatory chemokines (CCL2, CCL5, and IL12A) and NFκB-related molecule expression in BMDMs,

Who reads A20?

Author

He Li; Congpin Wang; Xiaoqing Li; Yinghui Kong; Weiguo Sun

Publisher

Hindawi Limited

Published

2021

Language

EN

Field

Medicine (Health Sciences)