About this Chemistry article
Optimization of solid-phase clean-up prior to liquid chromatographic analysis of ochratoxin A in roasted coffee by Liberty Sibanda; Sarah De Saeger; Carlos Van Peteghem is a Chemistry article available to read on EtoBox.
An extraction and clean-up method for ochratoxin A (OA) in roasted coffee has been developed and the HPLC method optimized. An interfering compound with a similar retention time as OA was adsorbed by the aminopropyl (NH2) material at < or = 5% NaHCO3. Residual OA on the column was recovered by washing with the extraction solution followed with methanol. Fractions were mixed together for further clean-up with Ochratest immunoaffinity columns (IACs). Analysis by HPLC resulted in a well resolved OA peak and reduction in matrix interferences. Recoveries ranged from 72 to 84% and the detection limit was 1 ng/g.
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Liberty Sibanda; Sarah De Saeger; Carlos Van Peteghem
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)