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The study investigates the primase activity of the Saccharomyces cerevisiae mitochondrial RNA polymerase (Rpo41) and its transcription factor (Mtf1) on single-stranded DNA (ssDNA). It demonstrates that the Rpo41-Mtf1 complex efficiently synthesizes RNA primers that initiate DNA synthesis, even in the presence of the ssDNA-binding protein Rim1, which inhibits Rpo41 alone. The findings suggest that Rpo41-Mtf1 is a more effective primase compared to Rpo41, facilitating both leading and lagging strand DNA synth
- Author
- Dat Le Mai Tan
- Language
- EN