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T-cell and B-cell repertoire diversity are selectively skewed in children with idiopathic nephrotic syndrome revealed by high-throughput sequencing by Qing Ye; Dong-Jie Wang; Bing Lan; Jian-Hua Mao is a Medicine article available to read on EtoBox.

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## Background: Clinical studies suggest that the dysfunction of t cells and b cells may play an essential role in the pathogenesis of idiopathic nephrotic syndrome (ins), but laboratory evidence is lacking. therefore, this study explored t-cell receptor (tcr) and b-cell receptor (bcr) profiling in children with idiopathic nephrotic syndrome. ## Methods: High-throughput sequencing technology was used to profile the tcr and bcr repertoires in children with ins. peripheral blood was collected from ten ins patients, including five vinculin autoantibody-positive patients and five vinculin autoantibody-negative patients, before and after treatment. tcr and bcr libraries were constructed by 5'-race and sequenced by a dnbseq-t7 sequencer, and sequence analyses were performed using reseqtools, fastp, mixcr, and vdjtools. ## Results: The tra (t-cell receptor α), trg (t-cell receptor γ), and igh (immunoglobulin heavy chain) repertoires of the ins group were occupied by highly abundant clonotypes, whereas small clonotypes occupied the healthy group, especially tra. a significant increase in the shannon-weaver index was observed for the tra and trg repertoires after treatment in vinculin autoan

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Author

Qing Ye; Dong-Jie Wang; Bing Lan; Jian-Hua Mao

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Medicine (Health Sciences)