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Nisin-loaded poly-l-lactide nano-particles produced by CO2 anti-solvent precipitation for sustained antimicrobial activity by Stefano Salmaso; Nicola Elvassore; Albero Bertucco; Anna Lante; Paolo Caliceti is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

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Nisin-loaded poly-l-lactide (PLA) nano-particles were fabricated by processing protein/polymer organic solutions by semicontinuous compressed CO 2 anti-solvent precipitation. Preliminary solubility studies were carried out for an optimised selection of organic solvent mixtures leading to preparation of protein/polymer solutions. The particles were prepared by processing 50:50 dimethylsulfoxide/dichloromethane mixtures containing 1% polymer (w/v) and 5 or 20% nisin (nisin/polymer, w/w). Proper operative conditions (organic solution injection rate, precipitation temperature and gas pressure, CO 2 flow rate, washing time, etc.) were set up to yield production reproducibility, high product recovery (over 70%) and high drug loading (over 95% of the recovered protein). Scanning electron microscopy demonstrated that spherical, smooth surfaced particles were produced. Light scattering showed that the particle size was in the range of 200-400 nm and the products were characterised by narrow polydispersity. In vitro release studies showed that the protein is slowly released throughout 1000 h. However, the release was slower as the salt concentration and the pH of the release buffer increased

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Author

Stefano Salmaso; Nicola Elvassore; Albero Bertucco; Anna Lante; Paolo Caliceti

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)

Published

2004

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)