Can I read Apolipoprotein E and Low‐Density Lipoprotein Binding and Internalization in Primary Cultures of Rat Astrocytes: Isoform‐Specific Alterations on EtoBox?

Apolipoprotein E and Low‐Density Lipoprotein Binding and Internalization in Primary Cultures of Rat Astrocytes: Isoform‐Specific Alterations by Daniel Guillaume; Philippe Bertrand; Doris Dea; Jean Davignon; Judes Poirier is a Neuroscience article available to read on EtoBox.

What is Apolipoprotein E and Low‐Density Lipoprotein Binding and Internalization in Primary Cultures of Rat Astrocytes: Isoform‐Specific Alterations about?

Abstract: Apolipoprotein (apo) E is likely involved in redistributing cholesterol and phospholipids during compensatory synaptogenesis in the injured CNS. Three common isoforms of apoE exist in human (E2, E3, and E4). The apoE4 allele frequency is markedly increased in both late‐onset sporadic and familial Alzheimer's disease (AD). ApoE concentration in the brain of AD subjects follows a gradient: ApoE levels decrease as a function of E2 > E3 ≫ E4. It has been proposed that the poor reinnervation capacity reported in AD may be caused by impairment of the apoE/low‐density lipoprotein (LDL) receptor activity. To understand further the role of this particular axis in lipid homeostasis in the CNS, we have characterized binding, internalization, and degradation of human ^125^I‐LDL to primary cultures of rat astrocytes. Specific binding was saturable, with a __K__~D~ of 1.8 n__M__ and a __B__~max~ of 0.14 pmol/mg of proteins. Excess unlabeled human LDL or very LDL (VLDL) displaced 70% of total binding. Studies at 37°C confirmed that astrocytes bind, internalize, and degrade ^125^I‐LDL by a specific, saturable mechanism. Reconstituted apoE (E2, E3, and E4)‐liposomes were labeled with ^125

Who reads Apolipoprotein E and Low‐Density Lipoprotein Binding and Internalization in Primary Cultures of Rat Astrocytes: Isoform‐Specific Alterations?

Author

Daniel Guillaume; Philippe Bertrand; Doris Dea; Jean Davignon; Judes Poirier

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1996

Language

EN

Field

Neuroscience (Life Sciences)