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CYP1 and AhR expression in 7,12-dimethylbenz[a]anthracene-induced mammary carcinoma of rats prenatally exposed to 3,3′,4,4′,5-pentachlorobiphenyl by Shin Wakui; Kiyofumi Yokoo; Hiroyuki Takahashi; Tomoko Muto; Yoshihiko Suzuki; Yoshikatsu Kanai; Hiroshi Hano; Masakuni Furusato; Hitoshi Endou is a Environmental Science article available to read on EtoBox.

What is CYP1 and AhR expression in 7,12-dimethylbenz[a]anthracene-induced mammary carcinoma of rats prenatally exposed to 3,3′,4,4′,5-pentachlorobiphenyl about?

We previously reported the finding that prenatal exposure to a relatively low dose of 3,3',4,4',5-pentachlorobiphenyl (PCB126) acted as an enhancing agent for 17-beta-estradiol (E2)-dependent 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary carcinoma, while a high dose decreased it. E2 is a known risk factor for mammary carcinoma, and CYP1A1 and 1B1 (CYP1) are the major enzymes catalyzing 2- and 4-hydroxylation of E2, respectively. We investigated the induction of CYP1 and aryl hydrocarbon receptor (AhR) in DMBA-induced mammary carcinoma using female Sprague-Dawley rats whose dams had been treated (i.g.) with 2.5 ng, 250 ng, 7.5 microg of PCB126/kg or the vehicle on days 13-19 post-conception. Immunohistochemical analysis revealed that the mammary carcinoma of the 250 ng group showed a significantly higher number of nuclei expressing estrogen receptor alpha (ER) and proliferating cell nuclear antigen (PCNA) compared to those of the other groups. Quantitative real-time RT-PCR analysis revealed that the 7.5 microg group showed a significantly higher level of CYP1A1 mRNA, and that the 250 ng group showed significantly higher levels of CYP1B1 mRNA. The level of AhR mRNA was si

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Author

Shin Wakui; Kiyofumi Yokoo; Hiroyuki Takahashi; Tomoko Muto; Yoshihiko Suzuki; Yoshikatsu Kanai; Hiroshi Hano; Masakuni Furusato; Hitoshi Endou

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)

Published

2005

Language

EN

Field

Environmental Science (Life Sciences)