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Bacteriophages are viruses that infect bacteria, consisting of DNA or RNA encased in a protein coat, and can undergo lytic or lysogenic cycles. Lambda and M13 phages are commonly used as cloning vectors, with lambda phages having limitations in DNA size insertion and restriction site issues, which can be addressed through genome modification. M13 phages are advantageous for cloning due to their smaller genome size and ability to produce single-stranded DNA, facilitating various genetic techniques.

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sohaibnawaz156
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