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Role of Alpha8 Integrin in Mesangial Cell Adhesion, Migration, and Proliferation by Bieritz, Beate; Spessotto, Paola; Colombatti, Alfonso; Jahn, Angelika; Prols, Felicitas; Hartner, Andrea is a Medicine article available to read on EtoBox.

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## Background: Extracellular matrix receptors of the integrin family are known to regulate cell adhesion, migration, and proliferation. the alpha8 integrin chain is expressed in the glomerulus exclusively by mesangial cells. the contribution of alpha8 to mesangial cell function, however, has not yet been studied. ## Methods: Mesangial cells from wild-type and alpha8-deficient mice were isolated and characterized. integrin expression was assessed by real-time polymerase chain reaction (pcr), western blot, or fluorescence-activated cell sorter (facs) analysis. cell adhesion was determined by conventional attachment assay and a centrifugal assay for cell adhesion. cell migration was determined by a fluorescence-based transmigration assay and a chemotaxis assay. proliferation rates were determined by brdu and [3h]-thymidine assays. ## Results: On the alpha8 ligands fibronectin and vitronectin, but not on collagens, attachment of alpha8-deficient mesangial cells was reduced compared to wild-type cells. in contrast, alpha8-deficient mesangial cells migrated more easily and displayed an increased proliferative response on fibronectin or vitronectin, but not on collagens, compared to wild-

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Author

Bieritz, Beate; Spessotto, Paola; Colombatti, Alfonso; Jahn, Angelika; Prols, Felicitas; Hartner, Andrea

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; ScienceOpen (ISSN 0085-2538)

Published

2003

Language

EN

Field

Medicine (Health Sciences)