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Giardia in a selected population of dogs and cats in Germany – diagnostics, coinfections and assemblages by M.F. Sommer; P. Rupp; M. Pietsch; A. Kaspar; P. Beelitz is a Immunology and Microbiology article available to read on EtoBox.

What is Giardia in a selected population of dogs and cats in Germany – diagnostics, coinfections and assemblages about?

Infections with the zoonotic endoparasite Giardia duodenalis are widely spread among dogs and cats worldwide. Since the question whether the infection might be transmitted from domestic animals to their owners is still an important topic, a reliable detection of patent Giardia infections and the determination of the associated Giardia assemblages is of major concern. The objectives of the present study were to determine the prevalence of Giardia infections in dogs and cats living in Germany using different diagnostic tests and to identify the Giardia assemblages of infected animals. Furthermore, a possible correlation of coinfections with other endoparasites was analysed. All samples were investigated by enzyme-linked immunosorbent assay (ELISA), merthiolate-iodine-formalin concentration technique (MIFC) and zinc chloride flotation. ELISA-positive samples were additionally screened with a direct immunofluorescence assay (IFA). Faecal DNA was extracted from all Giardia cyst-positive samples and used for multilocus sequence typing with nested PCRs targeting the following gene loci: SSU rRNA (SSU), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi). Samples from dogs an

Who reads Giardia in a selected population of dogs and cats in Germany – diagnostics, coinfections and assemblages?

Author

M.F. Sommer; P. Rupp; M. Pietsch; A. Kaspar; P. Beelitz

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)

Published

2017

Language

EN

Field

Immunology and Microbiology (Life Sciences)