Can I read Costunolide Causes Mitotic Arrest and Enhances Radiosensitivity in Human Hepatocellular Carcinoma Cells on EtoBox?

Costunolide Causes Mitotic Arrest and Enhances Radiosensitivity in Human Hepatocellular Carcinoma Cells by Chia-Yuan Liu; Hsun-Shuo Chang; Ih-Sheng Chen; Chih-Jen Chen; Ming-Ling Hsu; Shu-Ling Fu; Yu-Jen Chen is a Medicine article available to read on EtoBox.

What is Costunolide Causes Mitotic Arrest and Enhances Radiosensitivity in Human Hepatocellular Carcinoma Cells about?

## Purpose This work aimed to investigate the effect of costunolide, a sesquiterpene lactone isolated from __Michelia compressa__, on cell cycle distribution and radiosensitivity of human hepatocellular carcinoma (HCC) cells. ## Methods The assessment used in this study included: cell viability assay, cell cycle analysis by DNA histogram, expression of phosphorylated histone H3 (Ser 10) by flow cytometer, mitotic index by Liu's stain and morphological observation, mitotic spindle alignment by immunofluorescence of alpha-tubulin, expression of cell cycle-related proteins by Western blotting, and radiation survival by clonogenic assay. ## Results Our results show that costunolide reduced the viability of HA22T/VGH cells. It caused a rapid G2/M arrest at 4 hours shown by DNA histogram. The increase in phosphorylated histone H3 (Ser 10)-positive cells and mitotic index indicates costunolide-treated cells are arrested at mitosis, not G2, phase. Immunofluorescence of alpha-tubulin for spindle formation further demonstrated these cells are halted at metaphase. Costunolide up-regulated the expression of phosphorylated Chk2 (Thr 68), phosphorylated Cdc25c (Ser 216), phosphorylated Cdk1 (Tyr

Who reads Costunolide Causes Mitotic Arrest and Enhances Radiosensitivity in Human Hepatocellular Carcinoma Cells?

Author

Chia-Yuan Liu; Hsun-Shuo Chang; Ih-Sheng Chen; Chih-Jen Chen; Ming-Ling Hsu; Shu-Ling Fu; Yu-Jen Chen

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2006-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1748-717X)

Published

2011

Language

EN

Field

Medicine (Health Sciences)