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Changes in secreted and cell associated proteoglycan synthesis during conversion of myoblasts to osteoblasts in response to bone morphogenetic protein‐2: Role of decorin in cell response to BMP‐2 by Jaime Gutierrez; Nelson Osses; Enrique Brandan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Proteoglycans have been identified within the extracellular matrices (ECM) of bone and are known to play a role in ECM assembly, mineralization, and bone formation. Bone morphogenetic protein‐2 (BMP‐2) specifically converts the differentiation pathway of C2C12 myoblasts into that of osteoblast lineage cells. Microarray analyses of the mouse myoblast cell line C2C12 and its differentiation into osteoblastic cells in response to BMP‐2 have suggested the up‐regulation of several proteoglycan species, although there is a lack of biochemical evidence for this response. In this study we have biochemically analyzed and characterized the proteoglycan populations that are induced in C2C12 cells upon osteoblastic differentiation produced by BMP‐2. An important and specific increase in the synthesis of secreted decorin was observed in BMP‐2‐treated cells, as compared to untreated myoblasts and myoblasts induced to differentiate into myotubes. Decorin was seen to contain larger glycosaminoglycan (GAG) chains in induced than in non‐induced cells. BMP‐2 also produced an augment in the synthesis of different heparan sulfate proteoglycans such syndecan‐2, ‐ 3, glypican, and perlecan in

Who reads Changes in secreted and cell associated proteoglycan synthesis during conversion of myoblasts to osteoblasts in response to bone morphogenetic protein‐2: Role of decorin in cell response to BMP‐2?

Author

Jaime Gutierrez; Nelson Osses; Enrique Brandan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)