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Peptide immunization of guinea pigs against Chlamydia psittaci (GPIC agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge by K Volp; S Mathews; P Timms; LM Hafner is a Immunology and Microbiology article available to read on EtoBox.

What is Peptide immunization of guinea pigs against Chlamydia psittaci (GPIC agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge about?

Immunization of female guinea pigs with a chimeric peptide consisting of variable domain IV (VDIV) and a region known as GP8 from the major outer membrane protein of __Chlamydophila caviae__, formerly __Chlamydia psittaci__ guinea pig inclusion conjunctivitis strain, was performed to assess whether humoral immune responses could be elicited in the reproductive tracts of immunized animals. The __C. caviae__ strain is able to cause a sexually transmitted infection in the guinea pig that closely parallels __C. trachomatis__ infections in humans. The best anti‐VDIV antibody response in vaginal secretions was achieved by intraperitoneal priming with subsequent intravaginal boosting (__P__ < 0.001). Dot‐blot analyses of vaginal secretions confirmed that these anti‐VDIV antibodies, produced against a linear peptide, were able to recognize and bind to whole conformational __C. caviae__ elementary bodies. Following live intravaginal challenge with __C. caviae__, a significant reduction in the intensity (__P__ = 0.01) and an apparent reduction in the duration of the infection was evident between the guinea pigs immunized with VDIV‐GP8 and non‐immunized controls.

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Author

K Volp; S Mathews; P Timms; LM Hafner

Publisher

Nature Publishing Group; Wiley (Blackwell Publishing); John Wiley and Sons Inc.; Wiley (ISSN 0818-9641)

Published

2001

Language

EN

Field

Immunology and Microbiology (Life Sciences)