Opening book details…
Can I read Bax Ablation Protects Against Hepatic Ischemia/reperfusion Injury in Transgenic Mice on EtoBox?
Bax Ablation Protects Against Hepatic Ischemia/reperfusion Injury in Transgenic Mice by Ziv Ben-Ari; Orit Pappo; Yelena Cheporko; Natali Yasovich; Daniel Offen; Asher Shainberg; Dorit Leshem; Jacquelin Sulkes; Bernardo A. Vidne; Edith Hochhauser is a Medicine article available to read on EtoBox.
What is Bax Ablation Protects Against Hepatic Ischemia/reperfusion Injury in Transgenic Mice about?
Apoptosis appears to be a central mechanism of cell death following reperfusion of the ischemic liver. The aim of this study was to determine the effect of decreased expression of the proapoptotic Bax gene on hepatic apoptotic warm ischemia/reperfusion (I/R) injury. Three groups of mice were studied: homozygotic knockout mice (Bax Ϫ/Ϫ ); heterozygotic (Bax ϩ/Ϫ ); and wild type (Bax ϩ/ϩ ). Isolated mouse livers were subjected to 90 minutes of ischemia (37°C) followed by 15 minutes of reperfusion. Bax and Bcl-2 expression in liver tissue homogenates was measured by Western blot. Serum liver enzyme levels were measured and intrahepatic caspase-3 activity was determined by fluorimetric assay. Oil red O (ORO) staining was performed for fat detection. Apoptotic cells were identified by morphological criteria, immunohistochemistry for caspase-3, and terminal deoxynucleotidyl transferase-mediated 2Ј-deoxyuridine 5Ј-triphosphate nick-end labeling (TUNEL) assay. At 1 minute of reperfusion, the ischemic (Bax Ϫ/Ϫ ) livers were characterized by statistically significantly lower liver enzyme levels and lower caspase-3 activity than the ischemic (Bax ϩ/ϩ ) livers (P Ͻ 0.05 for both). The reductio
Who reads Bax Ablation Protects Against Hepatic Ischemia/reperfusion Injury in Transgenic Mice?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Ziv Ben-Ari; Orit Pappo; Yelena Cheporko; Natali Yasovich; Daniel Offen; Asher Shainberg; Dorit Leshem; Jacquelin Sulkes; Bernardo A. Vidne; Edith Hochhauser
- Publisher
- Ovid Technologies (Wolters Kluwer Health)
- Published
- 2007
- Language
- EN
- Field
- Medicine (Health Sciences)