Can I read MotX and MotY, specific components of the sodium‐driven flagellar motor, colocalize to the outer membrane in Vibrio alginolyticus on EtoBox?
MotX and MotY, specific components of the sodium‐driven flagellar motor, colocalize to the outer membrane in Vibrio alginolyticus by Mayuko Okabe; Toshiharu Yakushi; Masaru Kojima; Michio Homma is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is MotX and MotY, specific components of the sodium‐driven flagellar motor, colocalize to the outer membrane in Vibrio alginolyticus about?
## Summary Rotation of the sodium‐driven polar flagella of __Vibrio alginolyticus__ requires four motor proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium‐driven motor of __Vibrio__, have been believed to be localized in the inner (cytoplasmic) membrane via their N‐terminal hydrophobic segments. Here we show that MotX and MotY colocalize to the outer membrane. Both proteins, when expressed together, were detected in the outer membrane fraction separated by sucrose density gradient centrifugation. As mature MotX and MotY proteins do not have N‐terminal hydrophobic segments, the N‐termini of the primary translation products must have signal sequences that are removed upon translocation across the inner membrane. Moreover, MotX and MotY require each other for efficient localization to the outer membrane. Based on these lines of evidence, we propose that MotX and MotY form a complex in the outer membrane. This is the first case that describes motor proteins function in the outer membrane for flagellar rotation.
Who reads MotX and MotY, specific components of the sodium‐driven flagellar motor, colocalize to the outer membrane in Vibrio alginolyticus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mayuko Okabe; Toshiharu Yakushi; Masaru Kojima; Michio Homma
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)