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333 pages : 25 cm Includes bibliographical references and index 1. High performance liquid chromatography of proteins and nucleic acids / Keith Gooderham -- 2. Analytical electrophoretic techniques in protein chemistry / Bryan John Smith and Robert H. Nicolas -- 3. Protein blotting / Keith Gooderham -- 4. The production and isolation of peptides for sequence analysis / John M. Walker and Elaine L.V. Mayes -- 5. Protein and peptide sequence determination / John M. Walker -- 6. The extraction and fractionation of RNA / Robert J. Slater -- 7. Protein synthesis in vitro / Don Grierson and Jim Speirs -- 8. Labelling DNA in vitro: nick translation / Christopher G.P. Mathew -- 9. Synthesis of cDNA for molecular cloning / Brian G. Forde -- 10. Plasmid isolation / Stephen A. Boffey -- 11. The use of restriction endonucleases and T4 DNA ligase / Frits R. Mooi and Wim Gaastra -- 12. Molecular cloning of cDNA: bacterial transformation and screening of transformants / Brian G. Forde -- 13. The use of minicells and maxicells to detect the expression of cloned genes / Bauke Oudega and Frits R. Mooi -- 14. Gel electrophoresis of DNA / Stephen A. Boffey -- 15. Detection of specific DNA sequences: t
- Author
- John M. Walker and Wim Gaastra
- Publisher
- Macmillan Pub Co
- Published
- 1983
- Language
- EN
- ISBN
- 9780029498309
- Subjects
- Science, Engineering, Biology
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