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Transient Kinetics of Hybridoma Growth and Monoclonal Antibody Production in Serum-limited Cultures by Mina Dalili; David F. Ollis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2-10°/0 FCS), giving K,,, = 1.6 v/v% and pmax = 0.92 d-'. The maximum cell yields (total and viable) were linear with initial serum level, indicating stoichiometric as well as kinetic limitation by serum component(s). The specific antibody production rate for each individual run fitted well to a non-growth-associated model. However, the non-growthassociated parameter varied monotonically with initial serum concentration, suggesting the catalytic role of serum component(s) in antibody production. Also, glutamine was utilized inefficiently, with at least a third of it secreted back into the culture supernate in the form of glutamate. While very simple model equations describe the specific growth and product formation rate for an individual batch run, the larger picture indicates need for a more detailed unstructured or structured model.

Who reads Transient Kinetics of Hybridoma Growth and Monoclonal Antibody Production in Serum-limited Cultures?

Author

Mina Dalili; David F. Ollis

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)