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Analysis of Independence Based on Ice Nucleation Events Among Microplate Wells by Dimitris Zaragotas; Elias Anastassopoulos is a Medicine article available to read on EtoBox.

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In an earlier work, it was shown that non-isothermal conditions prevail during multiple freezing processes, e.g freezing in microplate wells. These non-isothermal conditions are due to either systematic or non-systematic temperature variations. The former are due to the position of the samples in the microplate, whereas the latter are due to the release of latent heat of freezing, which diffuses from frozen to adjacent samples changing their cooling rate, a phenomenon we described as neighbouring effect. In order to study the non-systematic temperature variations, we used an approach, adapted from previous studies with animal cells, which was based on the discrimination between independent and dependent nucleation events. In independent nucleations there were not any frozen adjacent wells present, whereas in dependent nucleations, there was at least one adjacent frozen well. The result of the analysis of independence which involved water, Taxus bacata, an evergreen shrub and Hippophae rhamonides, a deciduous shrub, proved that in the latter case, wells with three or four frozen adjacent wells froze at statistically significant warmer temperatures, than wells with one or two adjacen

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Author

Dimitris Zaragotas; Elias Anastassopoulos

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Medicine (Health Sciences)